Structural models for the design of novel antiviral agents against Spondweni virus helicase

Spondweni virus (SPONV), a possible emerging virus, is member of the Flaviviridae family, genus Flavivirus and belongs to serogroup with Zika virus. The latest epidemic fever, which broke out in Western Hemisphere, warns about risk corresponding urban potential SPONV calls for design anti‑SPONV therapies. Previous studies have demonstrated that viral RNA helicases represent promising pharmacological targets antiviral drugs/inhibitors, as they are implicated replication proliferation. Therefore, present study proposes three‑dimensional structure helicase/protease enzyme through homology modeling, using crystal Dengue virus‑4 same family template. For evaluation accuracy reliability model structure‑based drug strategies, hepatitis C (HCV) helicase was used, complexed single‑stranded RNA, key molecule establishment interactions future inhibitor helicase. Following identification motifs characteristic data from previous publications on treatment HCV were incorporated, aim detecting ideal residues model, similar those targets. existing pharmacophoric tested its application functionality use experiments novel agents.